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CDC6 (phospho S54) monoclonal antibody, clone HIB-3 

  • Catalog # : MAB20338
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic phosphopeptide of human CDC6.
  • Immunogen:
  • A synthetic phosphopeptide corresponding to residues surrounding S54 of human CDC6.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Western Blot (1:500-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of (1) Raji cell lysate; (2) Raji + FBS cell lysate with CDC6 (phospho S54) monoclonal antibody.
  • Immunocytochemistry
  • Immunofluorescence
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 990
  • Gene Name:
  • CDC6
  • Gene Alias:
  • CDC18L,HsCDC18,HsCDC6
  • Gene Description:
  • cell division cycle 6 homolog (S. cerevisiae)
  • Gene Summary:
  • The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq
  • Other Designations:
  • CDC18 (cell division cycle 18, S.pombe, homolog)-like,CDC6 cell division cycle 6 homolog,CDC6-related protein,cell division cycle 6 protein
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  • Interactome
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