CDC37 (phospho S13) monoclonal antibody, clone HIF-3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CDC37 (phospho S13) expression in (1) Jurkat cell lysate treated with Alkaline Phosphatase; (2) Jurkat cell lysate.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human CDC37.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S13 of human CDC37.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CDC37 (phospho S13) expression in (1) Jurkat cell lysate treated with Alkaline Phosphatase; (2) Jurkat cell lysate.Immunoprecipitation
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Gene Info — CDC37
Entrez GeneID
11140Protein Accession#
Q16543Gene Name
CDC37
Gene Alias
P50CDC37
Gene Description
cell division cycle 37 homolog (S. cerevisiae)
Omim ID
605065Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq
Other Designations
CDC37 (cell division cycle 37, S. cerevisiae, homolog)|CDC37 cell division cycle 37 homolog|Hsp90 co-chaperone Cdc37|cell division cycle 37 protein
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Interactome
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Disease
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