ATF2 (phospho T71) monoclonal antibody, clone FOO-1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of ATF2 (phospho T71) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human ATF2.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding T71 of human ATF2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:30)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ATF2 (phospho T71) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — ATF2
Entrez GeneID
1386Protein Accession#
P15336Gene Name
ATF2
Gene Alias
CRE-BP1, CREB2, HB16, MGC111558, TREB7
Gene Description
activating transcription factor 2
Omim ID
123811Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000163262|activating transcription factor 2 splice variant ATF2-var2|cAMP responsive element binding protein 2, formerly
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Interactome
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Pathway
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Disease
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