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Last updated: 2020/8/9
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PPIB monoclonal antibody, clone k2E2 

  • Catalog # : MAB2033
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant PPIB.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 26-216 of human PPIB.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1, kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: K562 cell lysate, Lane 3: 293T cell lysate, Lane 4: A549 cell lysate, Lane 5: MCF7 cell lysate, Lane 6: SK-OV-3 cell lysate, Lane 7: LnCap cell lysate.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of Hep3B cells. The cell was stained with PPIB monoclonal antibody, clone k2E2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of Hep3B cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 5479
  • Gene Name:
  • PPIB
  • Gene Alias:
  • CYP-S1,CYPB,MGC14109,MGC2224,SCYLP
  • Gene Description:
  • peptidylprolyl isomerase B (cyclophilin B)
  • Gene Summary:
  • The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. [provided by RefSeq
  • Other Designations:
  • PPIase,S-cyclophilin,cyclophilin B,cyclophilin-like protein,peptidyl-prolyl cis-trans isomerase B,peptidylprolyl isomerase B,rotamase
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