LEFTY2 monoclonal antibody, clone 7C5G1H6H10 (Cat # MAB2032) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY2. Detection occurs after 1.0 ug of protein is loaded in each lane. The blot was incubated with a 1:2,000 dilution of LEFTY2 monoclonal antibody, clone 7C5G1H6H10 at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1 : 1,000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. The antibody may be used to detect endogenous human LEFTY.
This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. The protein may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq