EGLN2 monoclonal antibody, clone ABD-5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell lysate; (2) A549 cell lysate with EGLN2 monoclonal antibody.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human EGLN2.
Immunogen
A synthetic peptide corresponding to human EGLN2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell lysate; (2) A549 cell lysate with EGLN2 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — EGLN2
Entrez GeneID
112398Protein Accession#
Q96KS0Gene Name
EGLN2
Gene Alias
DKFZp434E026, EIT6, HIFPH1, HPH-3, PHD1
Gene Description
egl nine homolog 2 (C. elegans)
Omim ID
606424Gene Ontology
HyperlinkGene Summary
The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this posttranslational modification. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
EGL nine (C.elegans) homolog 2|HIF prolyl hydroxylase 1|HIF-prolyl hydroxylase 1|estrogen-induced tag 6|hypoxia-inducible factor prolyl hydroxylase 1|prolyl hydroxylase domain-containing protein 1
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Interactome
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