PGK1 monoclonal antibody, clone EAB-16
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human PGK1.
Immunogen
A synthetic peptide corresponding to human PGK1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HepG2 cell lysate; (2) Mouse kidney lysate with PGK1 monoclonal antibody.Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — PGK1
Entrez GeneID
5230Protein Accession#
P00558Gene Name
PGK1
Gene Alias
MGC117307, MGC142128, MGC8947, MIG10, PGKA
Gene Description
phosphoglycerate kinase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq
Other Designations
OTTHUMP00000023595|cell migration-inducing gene 10 protein|primer recognition protein 2
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Glycolysis / Gluconeogenesis
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