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Last updated: 2021/8/8
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PCNA monoclonal antibody, clone DO-16 

  • Catalog # : MAB20300
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human PCNA.
  • Immunogen:
  • A synthetic peptide corresponding to human PCNA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Flow Cytometry (1:20)
    Immunocytochemistry (1:100-1:500)
    Immunofluorescence (1:100-1:500)
    Immunohistochemistry (1:50-1:200)
    Immunoprecipitation (1:50)
    Western Blot (1:3000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of (1) Hela cell lysate; (2) HepG2 whole cell lysate; (3) U937 whole cell lysate; (4) Mouse spleen lysate with PCNA monoclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining of paraffin-embedded human colon with PCNA monoclonal antibody.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 5111
  • Gene Name:
  • PCNA
  • Gene Alias:
  • MGC8367
  • Gene Description:
  • proliferating cell nuclear antigen
  • Gene Summary:
  • The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq
  • Other Designations:
  • DNA polymerase delta auxiliary protein,OTTHUMP00000030189,OTTHUMP00000030190,cyclin
  • Interactome
  • Interactome
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