MUC1 monoclonal antibody, clone FHA-13
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of T47D cell lysate with MUC1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human ovarian cancer with MUC1 monoclonal antibody.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human MUC1.
Immunogen
A synthetic peptide corresponding to human MUC1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:20)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of T47D cell lysate with MUC1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human ovarian cancer with MUC1 monoclonal antibody.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — MUC1
Entrez GeneID
4582Protein Accession#
P15941Gene Name
MUC1
Gene Alias
CD227, EMA, H23AG, MAM6, PEM, PEMT, PUM
Gene Description
mucin 1, cell surface associated
Omim ID
158340Gene Ontology
HyperlinkGene Summary
This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. [provided by RefSeq
Other Designations
DF3 antigen|H23 antigen|MUC-1/SEC|MUC-1/X|MUC1/ZD|OTTHUMP00000033787|OTTHUMP00000033790|OTTHUMP00000033795|breast carcinoma-associated antigen DF3|episialin|mucin 1|mucin 1, transmembrane|peanut-reactive urinary mucin|polymorphic epithelial mucin|tumor as
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Interactome
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Disease
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