MIF monoclonal antibody, clone ABAG-13
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human MIF.
Immunogen
A synthetic peptide corresponding to human MIF.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) THP-1 cell lysate; (2) Mouse brain lysate with MIF monoclonal antibody.Immunoprecipitation
Flow Cytometry
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Gene Info — MIF
Entrez GeneID
4282Protein Accession#
P14174Gene Name
MIF
Gene Alias
GIF, GLIF, MMIF
Gene Description
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Gene Ontology
HyperlinkGene Summary
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq
Other Designations
glycosylation-inhibiting factor|phenylpyruvate tautomerase
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Interactome
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Disease
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