HMGB1 monoclonal antibody, clone AAC-8
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) HeLa cell lysate, (2) Rat brain lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human HMGB1.
Immunogen
A synthetic peptide corresponding to human HMGB1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:30)
Immunocytochemistry (1:50-100)
Immunofluorescence (1:50-100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) HeLa cell lysate, (2) Rat brain lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human kidney.Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — HMGB1
Entrez GeneID
3146Protein Accession#
P09429Gene Name
HMGB1
Gene Alias
DKFZp686A04236, HMG1, HMG3, SBP-1
Gene Description
high-mobility group box 1
Omim ID
163905Gene Ontology
HyperlinkOther Designations
Amphoterin|OTTHUMP00000018199|OTTHUMP00000190860|Sulfoglucuronyl carbohydrate binding protein|high mobility group box 1|high mobility group protein 1|high-mobility group (nonhistone chromosomal) protein 1
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Interactome
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Pathway
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Disease
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