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Last updated: 2021/8/8

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HIST1H4A monoclonal antibody, clone HDC-8 

  • Catalog # : MAB20165
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human HIST1H4A.
  • Immunogen:
  • A synthetic peptide corresponding to human HIST1H4A.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ChIP
    Flow Cytometry (1:50)
    Immunoprecipitation (1:50)
    Immunocytochemistry (1:50-200)
    Immunofluorescence (1:50-200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-500)
    Western Blot (1:500-1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • Western Blot
  • Western Blot
  • Western Blot analysis of (1) HeLa, (2) 3T3 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Application Image
  • ChIP
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • Gene Information
  • Entrez GeneID:
  • 8359
  • Gene Name:
  • HIST1H4A
  • Gene Alias:
  • H4/a,H4FA
  • Gene Description:
  • histone cluster 1, H4a
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
  • Other Designations:
  • H4 histone family, member A,histone 1, H4a
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