H2AFZ monoclonal antibody, clone EOO-8
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of H2AFZ.
Immunogen
A synthetic peptide corresponding to H2AFZ.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Neuro-2a and Lane 2: HeLa cell lysates with H2AFZ monoclonal antibody, clone EOO-8 (Cat # MAB20145).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with H2AFZ monoclonal antibody, clone EOO-8 (Cat # MAB20145).Immunocytochemistry
Immunofluorescence
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Gene Info — H2AFZ
Entrez GeneID
3015Protein Accession#
P0C0S5Gene Name
H2AFZ
Gene Alias
H2A.z, H2A/z, H2AZ, MGC117173
Gene Description
H2A histone family, member Z
Omim ID
142763Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq
Other Designations
H2AZ histone
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Interactome
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Pathway
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