H2AFX monoclonal antibody, clone BIA-8
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Raji cell lysate with H2AFX monoclonal antibody, clone BIA-8 (Cat # MAB20144).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of rat lung with H2AFX monoclonal antibody, clone BIA-8 (Cat # MAB20144).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of H2AFX.
Immunogen
A synthetic peptide corresponding to H2AFX.
Host
Rabbit
Theoretical MW (kDa)
15.145
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Raji cell lysate with H2AFX monoclonal antibody, clone BIA-8 (Cat # MAB20144).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of rat lung with H2AFX monoclonal antibody, clone BIA-8 (Cat # MAB20144).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — H2AFX
Entrez GeneID
3014Protein Accession#
P16104Gene Name
H2AFX
Gene Alias
H2A.X, H2A/X, H2AX
Gene Description
H2A histone family, member X
Omim ID
601772Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
Other Designations
H2AX histone
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Interactome
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Pathway
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Disease
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