HDAC8 monoclonal antibody, clone HIO-8
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of HeLa cell lysate with HDAC8 monoclonal antibody, clone HIO-8 (Cat # MAB20130).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human HDAC8.
Immunogen
A synthetic peptide corresponding to human HDAC8.
Host
Rabbit
Theoretical MW (kDa)
41.758
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:30)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:30)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HeLa cell lysate with HDAC8 monoclonal antibody, clone HIO-8 (Cat # MAB20130).Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — HDAC8
Entrez GeneID
55869Protein Accession#
Q9BY41Gene Name
HDAC8
Gene Alias
HDACL1, RPD3
Gene Description
histone deacetylase 8
Omim ID
300269Gene Ontology
HyperlinkGene Summary
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. [provided by RefSeq
Other Designations
histone deacetylase-like 1
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Interactome
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Disease
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