HDAC6 monoclonal antibody, clone HEE-8
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human HDAC6.
Immunogen
A synthetic peptide corresponding to human HDAC6.
Host
Rabbit
Theoretical MW (kDa)
131.419
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:100-1:500)
Immunofluorescence (1:100-1:500)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:5000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HeLa cell lysate with HDAC6 monoclonal antibody, clone HEE-8 (Cat # MAB20127).Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — HDAC6
Entrez GeneID
10013Protein Accession#
Q9UBN7Gene Name
HDAC6
Gene Alias
FLJ16239, HD6, JM21
Gene Description
histone deacetylase 6
Omim ID
300272Gene Ontology
HyperlinkGene Summary
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq
Other Designations
OTTHUMP00000032398
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Interactome
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Disease
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