RIT1 monoclonal antibody, clone 14G7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis using RIT1 monoclonal antibody, clone 14G7 (Cat # MAB2011) on control 293 cells (lane 1), 293 cells expressing HA-tagged RIT1 protein (lane 2) and 293 cells expressing HA-tagged RIT2 protein (3).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant RIT1.
Immunogen
Recombinant protein corresponding to full length human RIT1.
Host
Mouse
Reactivity
Human, Mouse
Specificity
This antibody detects recombinant Rit protein. Does not cross-react with Rin protein.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western blot (0.5 to 1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM sodium phosphate buffer, 150 mM NaCl, pH 7.5 (50% glycerol, 3 mM sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis using RIT1 monoclonal antibody, clone 14G7 (Cat # MAB2011) on control 293 cells (lane 1), 293 cells expressing HA-tagged RIT1 protein (lane 2) and 293 cells expressing HA-tagged RIT2 protein (3). -
Gene Info — RIT1
Entrez GeneID
6016Gene Name
RIT1
Gene Alias
MGC125864, MGC125865, RIBB, RIT, ROC1
Gene Description
Ras-like without CAAX 1
Omim ID
609591Gene Ontology
HyperlinkGene Summary
RIT belongs to the RAS (HRAS; MIM 190020) subfamily of small GTPases (Hynds et al., 2003 [PubMed 12668729]).[supplied by OMIM
Other Designations
GTP-binding protein Roc1|OTTHUMP00000015932|Ric-like, expressed in many tissues
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Interactome
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Publication Reference
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Rit, a non-lipid-modified Ras-related protein, transforms NIH3T3 cells without activating the ERK, JNK, p38 MAPK or PI3K/Akt pathways.
Rusyn EV, Reynolds ER, Shao H, Grana TM, Chan TO, Andres DA, Cox AD.
Oncogene 2000 Sep; 19(41):4685.
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Biochemical characterization of the Ras-related GTPases Rit and Rin.
Shao H, Kadono-Okuda K, Finlin BS, Andres DA.
Archives of Biochemistry and Biophysics 1999 Nov; 371(2):207.
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Rin, a neuron-specific and calmodulin-binding small G-protein, and Rit define a novel subfamily of ras proteins.
Lee CH, Della NG, Chew CE, Zack DJ.
Journal of Neuroscience 1996 Nov; 16(21):6784.
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Rit, a non-lipid-modified Ras-related protein, transforms NIH3T3 cells without activating the ERK, JNK, p38 MAPK or PI3K/Akt pathways.
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