Rabbit monoclonal antibody raised against synthetic peptide of human HSPA5.
Immunogen:
A synthetic peptide corresponding to human HSPA5.
Host:
Rabbit
Theoretical MW (kDa):
72.333
Reactivity:
Human
Form:
Liquid
Purification:
Affinity purification
Isotype:
IgG
Recommend Usage:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM
Other Designations:
Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD),OTTHUMP00000022124,heat shock 70kD protein 5 (glucose-regulated protein, 78kD),heat shock 70kDa protein 5