Firefly Luciferase monoclonal antibody, clone DAA-6
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of Firefly Luciferase.
Immunogen
A synthetic peptide corresponding to amino acids 4-20 of Firefly Luciferase .
Sequence
AKNIKKGPAPFYPLEDG
Host
Rabbit
Theoretical MW (kDa)
60.745
Reactivity
Firefly
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Firefly Luciferase transfected 293T, Lane 2: HeLa, Lane 3: NIH/3T3 and Lane 4: C6 cell lysates with Firefly Luciferase monoclonal antibody, clone DAA-6 (Cat # MAB20045).Immunocytochemistry
Immunofluorescence
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