FBL monoclonal antibody, clone DIO-6
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human FBL.
Immunogen
A synthetic peptide corresponding to human FBL.
Host
Rabbit
Theoretical MW (kDa)
33.784
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Immunoprecipitation (1:30)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of HepG2 cell lysate with FBL monoclonal antibody, clone DIO-6 (Cat # MAB20042).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with FBL monoclonal antibody, clone DIO-6 (Cat # MAB20042).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — FBL
Entrez GeneID
2091Protein Accession#
P22087Gene Name
FBL
Gene Alias
FIB, FLRN, RNU3IP1
Gene Description
fibrillarin
Omim ID
134795Gene Ontology
HyperlinkGene Summary
This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq
Other Designations
34-kD nucleolar scleroderma antigen|RNA, U3 small nucleolar interacting protein 1
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Interactome
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Disease
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