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GFP monoclonal antibody, clone 9F9.F9

  • Catalog # : MAB2003
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant GFP.
  • Immunogen:
  • Recombinant GST fusion protein corresponding to full length jellyfish Aequorea victoria GFP.
  • Host:
  • Mouse
  • Reactivity:
  • Jellyfishes
  • Specificity:
  • Reactivity is observed against wild-type and recombinant forms of GFP. No reaction is seen against RFP.
  • Form:
  • Liquid
  • Isotype:
  • IgG, gamma 1
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:200000)
    Western Blot (1:3000-1:30000)
    Immunohistochemistry (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot of GFP recombinant protein detected with GFP monoclonal antibody (Cat # MAB2003).
    GFP recombinant protein was expressed in HeLa cells, where 50 ng (Lane 1), 100 ng (Lane 2) and 500 ng (Lane 3) of lysate were loaded per lane.
    GFP monoclonal antibody (Cat # MAB2003) detects a 27 KDa band corresponding to the epitope tag GFP.
    The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl.
    A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE.
    The protein was transferred to nitrocellulose using standard methods.
    After blocking with 5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 1 h at room temperature followed by washes and reaction with a 1 : 2,500 dilution of IRDye® 800 conjugated Goat-a-Mouse IgG [H&L] MX10.
    IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunohistochemistry
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry
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