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Last updated: 2022/7/3

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ENO1 monoclonal antibody, clone AEA-5 

  • Catalog # : MAB20011
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human ENO1.
  • Immunogen:
  • A synthetic peptide corresponding to human ENO1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 47.169
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunocytochemistry (1:50-1:200)
    Immunofluorescence (1:50-1:200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Immunoprecipitation (1:50)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western Blot analysis of Lane 1: MCF-7 whole cell and Lane 2: Rat brain tissue lysates with ENO1 monoclonal antibody, clone AEA-5 (Cat # MAB20011).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon with ENO1 monoclonal antibody, clone AEA-5 (Cat # MAB20011).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 2023
  • Gene Name:
  • ENO1
  • Gene Alias:
  • ENO1L1,MBP-1,MPB1,NNE,PPH
  • Gene Description:
  • enolase 1, (alpha)
  • Gene Summary:
  • This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq
  • Other Designations:
  • 2-phospho-D-glycerate hydro-lyase,MYC promoter-binding protein 1,OTTHUMP00000001706,alpha enolase like 1,enolase 1,non-neural enolase,phosphopyruvate hydratase,tau-crystallin
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