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Last updated: 2016/12/4
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Phosphothreonine monoclonal antibody, clone 18F6

  • Catalog # : MAB2001
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against Phosphothreonine.
  • Immunogen:
  • Phosphothreonine conjugated with KLH.
  • Host:
  • Mouse
  • Specificity:
  • Reactivity is specific to phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine.
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Quality Control Testing:
  • Antibody Reactive Against PhosphoThreonine.
  • Recommend Usage:
  • Competitive ELISA (1:2000-1:10000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Phospho Threonine monoclonal antibody, clone 18F6 (Cat # MAB2001) is shown to detect threonine phosphorylation of proteins in a lysate from A-431 cells stimulated with EGF.
    Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc.
    A 1 : 1,000 dilution of Phospho Threonine monoclonal antibody, clone 18F6 (Cat # MAB2001) is used for 30 min followed by detection using a 1 : 2,500 dilution of IRDye® 800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey® Infrared Imaging System developed by LI-COR.
    Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
  • ELISA
  • ELISA
  • ELISA results of PhosphoThreonine monoclonal antibody, clone 18F6 (Cat # MAB2001) tested against BSA conjugates of pT, pY and pS.
    Each well was coated with 0.1 ug of conjugate.
    The starting dilution of antibody was 1 : 1000 and each point on the X-axis represents a 2-fold dilution.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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