ELK1 monoclonal antibody, clone CHG-5
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Neuro-2a and Lane 2: K562 cell lysates with ELK1 monoclonal antibody, clone CHG-5 (Cat # MAB20009).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human ELK1.
Immunogen
A synthetic peptide corresponding to human ELK1.
Host
Rabbit
Theoretical MW (kDa)
44.888
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:30)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: Neuro-2a and Lane 2: K562 cell lysates with ELK1 monoclonal antibody, clone CHG-5 (Cat # MAB20009).Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — ELK1
Entrez GeneID
2002Protein Accession#
P19419Gene Name
ELK1
Gene Alias
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Gene Description
ELK1, member of ETS oncogene family
Omim ID
311040Gene Ontology
HyperlinkGene Summary
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
ELK1 protein|OTTHUMP00000023224|OTTHUMP00000023225
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Interactome
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Pathway
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Disease
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