TGFBI monoclonal antibody, clone EA-20
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of MCF7 cell lysate with TGFBI monoclonal antibody, clone EA-20 (Cat # MAB19997).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with TGFBI monoclonal antibody, clone EA-20 (Cat # MAB19997).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human TGFBI.
Immunogen
A synthetic peptide corresponding to human TGFBI.
Host
Rabbit
Theoretical MW (kDa)
74.681
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:5000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of MCF7 cell lysate with TGFBI monoclonal antibody, clone EA-20 (Cat # MAB19997).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with TGFBI monoclonal antibody, clone EA-20 (Cat # MAB19997).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — TGFBI
Entrez GeneID
7045Protein Accession#
Q15582Gene Name
TGFBI
Gene Alias
BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1
Gene Description
transforming growth factor, beta-induced, 68kDa
Gene Ontology
HyperlinkGene Summary
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq
Other Designations
RGD-containing collagen-associated protein|kerato-epithelin
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Interactome
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Disease
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