DNMT1 monoclonal antibody, clone AODB-4
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human DNMT1.
Immunogen
A synthetic peptide corresponding to human DNMT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HEK293 cell lysate; (2) NIH/3T3 cell lysate with DNMT1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
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Gene Info — DNMT1
Entrez GeneID
1786Protein Accession#
P26358Gene Name
DNMT1
Gene Alias
AIM, CXXC9, DNMT, FLJ16293, MCMT, MGC104992
Gene Description
DNA (cytosine-5-)-methyltransferase 1
Omim ID
126375Gene Ontology
HyperlinkGene Summary
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CXXC finger protein 9|DNA methyltransferase 1
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