GST monoclonal antibody, clone 3D4
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More Files
- More Functions
Western Blot
Western blot using GST monoclonal antibody, clone 3D4 (Cat # MAB1996) to detect purified GST recombinant protein (left) and in a lysate of HeLa cells expressing recombinant GST protein (right).
Approximately 0.1 ug of protein was separated by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of IRDye™800 conjugated Gt-a-Mouse IgG [H&L] MX.
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Specification
Product Description
Mouse monoclonal antibody raised against GST.
Immunogen
Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Host
Mouse
Reactivity
S. japonicum
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against GST-tag.
Recommend Usage
ELISA (1:20000-1:100000)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using GST monoclonal antibody, clone 3D4 (Cat # MAB1996) to detect purified GST recombinant protein (left) and in a lysate of HeLa cells expressing recombinant GST protein (right).
Approximately 0.1 ug of protein was separated by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of IRDye™800 conjugated Gt-a-Mouse IgG [H&L] MX.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.
Smith DB, Davern KM, Board PG, Tiu WU, Garcia EG, Mitchell GF.
PNAS 1986 Nov; 83(22):8703.
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Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.
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