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Last updated: 2016/12/4
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GST monoclonal antibody, clone 3D4

  • Catalog # : MAB1996
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against GST.
  • Immunogen:
  • Glutathione-S-Transferase (GST) from Schistosoma japonicum.
  • Host:
  • Mouse
  • Reactivity:
  • S. japonicum
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Quality Control Testing:
  • Antibody Reactive Against GST-tag.
  • Recommend Usage:
  • ELISA (1:20000-1:100000)
    Western Blot (1:1000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using GST monoclonal antibody, clone 3D4 (Cat # MAB1996) to detect purified GST recombinant protein (left) and in a lysate of HeLa cells expressing recombinant GST protein (right).
    Approximately 0.1 ug of protein was separated by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of IRDye™800 conjugated Gt-a-Mouse IgG [H&L] MX.
  • ELISA
  • Application Image
  • ELISA
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