MTBP monoclonal antibody, clone PHL-1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using MTBP monoclonal antibody, clone PHL-1 (Cat # MAB1995) shows detection of aband ~110 KDa corresponding to human MTBP (arrowhead).
Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct.
The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP.
Proteins were separated bySDS-PAGE and transferred onto PDVF membrane.
After blocking, the membrane was probed with the primary antibody diluted to 1 : 500 for 2h at roomtemperature followed by detection usinga Lumi-Light Plus Western Blotting Kit.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant MTBP.
Immunogen
Recombinant 6XHis fusion protein corresponding to amino acids 667-812 of human MTBP.
Host
Mouse
Reactivity
Chimpanzee, Human
Specificity
This antibody is directed against human MTBP protein. The product was produced from clarified mouse ascetic fluid.
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:2000-1:20000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using MTBP monoclonal antibody, clone PHL-1 (Cat # MAB1995) shows detection of aband ~110 KDa corresponding to human MTBP (arrowhead).
Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct.
The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP.
Proteins were separated bySDS-PAGE and transferred onto PDVF membrane.
After blocking, the membrane was probed with the primary antibody diluted to 1 : 500 for 2h at roomtemperature followed by detection usinga Lumi-Light Plus Western Blotting Kit.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MTBP
Entrez GeneID
27085Protein Accession#
Q96DY7;NP_071328Gene Name
MTBP
Gene Alias
MDM2BP
Gene Description
Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa
Omim ID
605927Gene Ontology
HyperlinkGene Summary
Mdm2
Other Designations
MDM2 (mouse double minute 2)-binding protein, 104kD|Mdm2, transformed 3T3 cell double minute 2, p53 binding protein binding protein
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Interactome
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Publication Reference
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Regulation of p53 and MDM2 activity by MTBP.
Brady M, Vlatkovic N, Boyd MT.
Molecular and Cellular Biochemistry 2005 Jan; 25(2):545.
Application：WB, Human, Mouse, H1299, MCF-7 cells, MEFs.
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A novel cellular protein (MTBP) binds to MDM2 and induces a G1 arrest that is suppressed by MDM2.
Boyd MT, Vlatkovic N, Haines DS.
The Journal of Biological Chemistry 2000 Oct; 275(41):31883.
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Assignment of the MDM2 binding protein gene (MTBP) to human chromosome band 8q24 by in situ hybridization.
Boyd MT, Zimonjic DB, Popescu NC, Athwal R, Haines DS.
Cytogenetics and Cell Genetics 2000 May; 90(1-2):64.
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Regulation of p53 and MDM2 activity by MTBP.
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