Hepatitis Virus gp1 monoclonal antibody, clone 2C6.H1
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More Files
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Western Blot
Western blotting using MHVgp1 monoclonal antibody, clone 2C6.H1 (Cat # MAB1994) to detect protein in various lysates, 6h post MHVgp1 infection.
Lane 1 shows no cross-reactivity with SARS-CoV-infected Vero cells.
Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown in lane 3.
Negative controls (lanes 2 and 4) show no staining.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
Immunofluorescence
Immunofluorescence microscopy using MHVgp1 monoclonal antibody, clone 2C6.H1 (Cat # MAB1994) , 6h post infection in mouse L cells.
Cells were fixed in 3% para-formaldehyde.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant MHVgp1.
Immunogen
Recombinant protein corresponding to full length mouse Hepatitis Virus (strain A59) MHVgp1.
Host
Mouse
Reactivity
Mouse
Specificity
This antibody is directed against the MHV-A59 nsp9 protein. No cross reactivity occurs with SARS CoV nsp9.
Form
Liquid
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:1000)
Immunofluorescence (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blotting using MHVgp1 monoclonal antibody, clone 2C6.H1 (Cat # MAB1994) to detect protein in various lysates, 6h post MHVgp1 infection.
Lane 1 shows no cross-reactivity with SARS-CoV-infected Vero cells.
Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown in lane 3.
Negative controls (lanes 2 and 4) show no staining.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.Immunofluorescence
Immunofluorescence microscopy using MHVgp1 monoclonal antibody, clone 2C6.H1 (Cat # MAB1994) , 6h post infection in mouse L cells.
Cells were fixed in 3% para-formaldehyde.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands. -
Gene Info — 1
Entrez GeneID
1489749Protein Accession#
NP_068668;MHV-A59Gene Name
1
Gene Alias
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Gene Description
ORF1ab polyprotein
Gene Ontology
HyperlinkOther Designations
ORF1a polyprotein
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Publication Reference
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The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world.
Egloff MP, Ferron F, Campanacci V, Longhi S, Rancurel C, Dutartre H, Snijder EJ, Gorbalenya AE, Cambillau C, Canard B.
PNAS 2004 Mar; 101(11):3792.
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The nsp9 replicase protein of SARS-coronavirus, structure and functional insights.
Sutton G, Fry E, Carter L, Sainsbury S, Walter T, Nettleship J, Berrow N, Owens R, Gilbert R, Davidson A, Siddell S, Poon LL, Diprose J, Alderton D, Walsh M, Grimes JM, Stuart DI.
Structure (London, England : 1993) 2004 Feb; 12(2):341.
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Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage.
Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, Poon LL, Guan Y, Rozanov M, Spaan WJ, Gorbalenya AE.
Journal of Molecular Biology 2003 Aug; 331(5):991.
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Four proteins processed from the replicase gene polyprotein of mouse hepatitis virus colocalize in the cell periphery and adjacent to sites of virion assembly.
Bost AG, Carnahan RH, Lu XT, Denison MR.
Journal of Virology 2000 Apr; 74(7):3379.
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Virus-encoded proteinases and proteolytic processing in the Nidovirales.
Ziebuhr J, Snijder EJ, Gorbalenya AE.
The Journal of General Virology 2000 Apr; 81(Pt 4):853.
Application：ELISA, IP, WB, Human, Human serum.
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The severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded RNA-binding subunit unique in the RNA virus world.
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