Mouse monoclonal antibody raised against native Keratin.
Native purified human Keratin.
Theoretical MW (kDa):
56, 46, 45, 40
This protein A purified mouse monoclonal antibody reacts specifically with keratins from human tissues and derived cell lines. This antibody reacts with keratin (56 KDa), keratin 17 (46 KDa), keratin 18 (45 KDa) and keratin 19 (40 KDa) derived from human. No reaction is expected against other filament proteins including vimentin, desmin and neurofilament protein.
Quality Control Testing:
Antibody Reactive Against Native Purified Protein.
The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using Keratin monoclonal antibody, clone C11 (Cat # MAB1991). This antibody recognizes asingle 56 KDa band corresponding to human Keratin as confirmed by the position of molecular weight markers (notshown). Approximatley 100 ng of keratin from human epidermis (Sigma p/n K0253) was applied under reducing conditions to a pre-cast 4-20% iGel from Gradipore Inc. A 1 : 400 dilution of Keratin monoclonal antibody, clone C11 was used for 2h followed by detection using a 1 : 5,000 dilution of IRDye™800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. IRDyeis a trademark of LI-COR, Inc.
Immunofluorescence microscopy using Keratin monoclonal antibody, clone C11 (Cat # MAB1991). Confocal slices of HeLa cells are between 0.5 and 0.6 um where the image is taken near the bottom of the cell.