Keratin monoclonal antibody, clone C11
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot using Keratin monoclonal antibody, clone C11 (Cat # MAB1991).
This antibody recognizes asingle 56 KDa band corresponding to human Keratin as confirmed by the position of molecular weight markers (notshown).
Approximatley 100 ng of keratin from human epidermis (Sigma p/n K0253) was applied under reducing conditions to a pre-cast 4-20% iGel from Gradipore Inc.
A 1 : 400 dilution of Keratin monoclonal antibody, clone C11 was used for 2h followed by detection using a 1 : 5,000 dilution of IRDye™800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDyeis a trademark of LI-COR, Inc.
Immunofluorescence
Immunofluorescence microscopy using Keratin monoclonal antibody, clone C11 (Cat # MAB1991).
Confocal slices of HeLa cells are between 0.5 and 0.6 um where the image is taken near the bottom of the cell.
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Specification
Product Description
Mouse monoclonal antibody raised against native Keratin.
Immunogen
Native purified human Keratin.
Host
Mouse
Theoretical MW (kDa)
56, 46, 45, 40
Reactivity
Human
Specificity
This protein A purified mouse monoclonal antibody reacts specifically with keratins from human tissues and derived cell lines. This antibody reacts with keratin (56 KDa), keratin 17 (46 KDa), keratin 18 (45 KDa) and keratin 19 (40 KDa) derived from human. No reaction is expected against other filament proteins including vimentin, desmin and neurofilament protein.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using Keratin monoclonal antibody, clone C11 (Cat # MAB1991).
This antibody recognizes asingle 56 KDa band corresponding to human Keratin as confirmed by the position of molecular weight markers (notshown).
Approximatley 100 ng of keratin from human epidermis (Sigma p/n K0253) was applied under reducing conditions to a pre-cast 4-20% iGel from Gradipore Inc.
A 1 : 400 dilution of Keratin monoclonal antibody, clone C11 was used for 2h followed by detection using a 1 : 5,000 dilution of IRDye™800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDyeis a trademark of LI-COR, Inc.Immunohistochemistry
Immunofluorescence
Immunofluorescence microscopy using Keratin monoclonal antibody, clone C11 (Cat # MAB1991).
Confocal slices of HeLa cells are between 0.5 and 0.6 um where the image is taken near the bottom of the cell.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.
Vojtĕsek B, Stasková Z, Nenutil R, Bártková J, Kovarík J, Rejthar A, Bártek J.
Neoplasma 1990 Jan; 37(3):333.
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Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread.
Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J.
International Journal of Cancer. Supplement 1988 Jan; 3:50.
Application：IHC-P, Human, Human bone-marrow samples, Human lymph-node specimens.
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The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
Moll R, Franke WW, Schiller DL, Geiger B, Krepler R.
Cell 1982 Nov; 31(1):11.
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A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.
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