CDC23 monoclonal antibody, clone IAA-3
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human CDC23.
Immunogen
A synthetic peptide corresponding to human CDC23.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1)HepG2 cell lysate; (2)Jurkat cell lysate with CDC23 monoclonal antibody.Immunocytochemistry
Immunofluorescence
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Gene Info — CDC23
Entrez GeneID
8697Protein Accession#
Q9UJX2Gene Name
CDC23
Gene Alias
APC8
Gene Description
cell division cycle 23 homolog (S. cerevisiae)
Omim ID
603462Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq
Other Designations
anaphase-promoting complex subunit 8|cell division cycle protein 23
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