CD247 monoclonal antibody, clone IHI-3
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More Files
- More Functions
Flow Cytometry
Flow cytometric analysis of mouse CD247 in splenocytes using 0.5 ug/10^6 cells. Green represents isotype control; red represents mouse CD247 antibody. Goat anti-hamster IgG FITC conjugate secondary antibody was used.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human CD247.
Immunogen
A synthetic peptide corresponding to human CD247.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:20)
Immunocytochemistry (1:100-1:200)
Immunofluorescence (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunofluorescence
Flow Cytometry
Flow cytometric analysis of mouse CD247 in splenocytes using 0.5 ug/10^6 cells. Green represents isotype control; red represents mouse CD247 antibody. Goat anti-hamster IgG FITC conjugate secondary antibody was used. -
Gene Info — CD247
Entrez GeneID
919Protein Accession#
P20963Gene Name
CD247
Gene Alias
CD3-ZETA, CD3H, CD3Q, CD3Z, T3Z, TCRZ
Gene Description
CD247 molecule
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD247 antigen, zeta subunit|CD3Z antigen, zeta polypeptide (TiT3 complex)|OTTHUMP00000032544|T-cell antigen receptor complex, zeta subunit of CD3|T-cell receptor T3 zeta chain|T-cell receptor zeta chain|T-cell surface glycoprotein CD3 zeta chain
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Interactome
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Pathway
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Disease
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