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ATF6 alpha monoclonal antibody, clone 37-1 

  • Catalog # : MAB19823
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant human ATF6 alpha.
  • Sequence:
  • Recombinant GST fusion protein corresponding to amino acids fragment at N-terminus of human ATF6 alpha.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody reacts to human and mouse ATF6 alpha.
  • Form:
  • Liquid
  • Purification:
  • Chromatography Method
  • Isotype:
  • IgG1 kappa
  • Recommend Usage:
  • Western Blot (1:500 - 1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol).
  • Storage Instruction:
  • Stored at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • (Right)
    Western Blot analysis of human Hela cell extracts: Lane 1: untreated; Lane 2: DTT: 1mM dithiothreitol (reducing reagent) for 1 h; Lane 3: Tm: 2 g/ml tunicamycin (inhibitor of N-glycosylation) for 3 h; Lane 4: Tm: 2 g/ml tunicamycin (inhibitor of N-glycosylation) for 7 h with ATF6 alpha monoclonal antibody, clone 37-1 (Cat# MAB19823).

    *The upper arrow denotes an unglycosylated form of pATF6 alpha (P); the bottom arrow denotes pATF6 alpha (N). ATF6 is constitutively expressed as pATF6 alpha (P) ( ~90kDa protein), and converted to pATF6 alpha (N) (>50-kDa protein) in ER-stressed cells.


    (Left)
    Western Blot analysis of mouse NIH3T3 cell extracts: Lane 1: untreated; Lane 2: DTT: 1mM dithiothreitol (reducing reagent) for 1 h; Lane 3: Tm: 2 g/ml tunicamycin (inhibitor of N-glycosylation) for 3 h; Lane 4: Tm: 2 g/ml tunicamycin (inhibitor of N-glycosylation) for 7 h with ATF6 alpha monoclonal antibody, clone 37-1 (Cat# MAB19823).

    *The upper arrow denotes an unglycosylated form of pATF6 alpha (P); the bottom arrow denotes pATF6 alpha (N). ATF6 is constitutively expressed as pATF6 alpha (P) ( ~90kDa protein), and converted to pATF6 alpha (N) (>50-kDa protein) in ER-stressed cells.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • ATF6
  • Gene Alias:
  • ATF6A
  • Gene Description:
  • activating transcription factor 6
  • Gene Summary:
  • ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000025585,activating transcription factor 6 alpha
  • Gene Pathway
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