(Right) Western Blot analysis of human Hela cell extracts: Lane 1: untreated; Lane 2: DTT: 1mM dithiothreitol (reducing reagent) for 1 h; Lane 3: Tm: 2 g/ml tunicamycin (inhibitor of N-glycosylation) for 3 h; Lane 4: Tm: 2 g/ml tunicamycin (inhibitor of N-glycosylation) for 7 h with ATF6 alpha monoclonal antibody, clone 37-1 (Cat# MAB19823).
*The upper arrow denotes an unglycosylated form of pATF6 alpha (P); the bottom arrow denotes pATF6 alpha (N). ATF6 is constitutively expressed as pATF6 alpha (P) ( ~90kDa protein), and converted to pATF6 alpha (N) (>50-kDa protein) in ER-stressed cells.
(Left) Western Blot analysis of mouse NIH3T3 cell extracts: Lane 1: untreated; Lane 2: DTT: 1mM dithiothreitol (reducing reagent) for 1 h; Lane 3: Tm: 2 g/ml tunicamycin (inhibitor of N-glycosylation) for 3 h; Lane 4: Tm: 2 g/ml tunicamycin (inhibitor of N-glycosylation) for 7 h with ATF6 alpha monoclonal antibody, clone 37-1 (Cat# MAB19823).
*The upper arrow denotes an unglycosylated form of pATF6 alpha (P); the bottom arrow denotes pATF6 alpha (N). ATF6 is constitutively expressed as pATF6 alpha (P) ( ~90kDa protein), and converted to pATF6 alpha (N) (>50-kDa protein) in ER-stressed cells.
ATF6 is an endoplasmic reticulum (ER) stress-regulated transmembrane transcription factor that activates the transcription of ER molecules.[supplied by OMIM