CA9 monoclonal antibody, clone EFE-3
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More Files
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Western Blot (Tissue lysate)
Western Blot analysis of human stomach tissue lysate with CA9 monoclonal antibody, clone EFE-3 (Cat # MAB19682).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human CA9.
Immunogen
A synthetic peptide corresponding to human CA9.
Host
Rabbit
Theoretical MW (kDa)
49.698
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot analysis of human stomach tissue lysate with CA9 monoclonal antibody, clone EFE-3 (Cat # MAB19682).Immunoprecipitation
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Gene Info — CA9
Entrez GeneID
768Protein Accession#
Q16790Gene Name
CA9
Gene Alias
CAIX, MN
Gene Description
carbonic anhydrase IX
Omim ID
603179Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq
Other Designations
OTTHUMP00000022773|RCC-associated protein G250|carbonic dehydratase
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Interactome
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