NPDC1 monoclonal antibody, clone 16E4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot using NPDC1 monoclonal antibody, clone 16E4 (Cat # MAB1965) on 10 ug lysate transfected with NPDC1 protein at a dilution of 1 ug/mL .
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of NPDC1.
Immunogen
A synthetic peptide corresponding to human NPDC1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM sodium phosphate buffer, 150 mM NaCl, pH 7.5 (50% glycerol, 3 mM sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using NPDC1 monoclonal antibody, clone 16E4 (Cat # MAB1965) on 10 ug lysate transfected with NPDC1 protein at a dilution of 1 ug/mL .Immunohistochemistry
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Gene Info — NPDC1
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Interactome
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Publication Reference
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Subcellular localization of neural-specific NPDC-1 protein.
Evrard C, Rouget P.
Journal of Neuroscience Research 2005 Mar; 79(6):747.
Application：WB-Ti, Rat , Rat brains.
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NPDC-1, a novel regulator of neuronal proliferation, is degraded by the ubiquitin/proteasome system through a PEST degradation motif.
Spencer ML, Theodosiou M, Noonan DJ.
The Journal of Biological Chemistry 2004 Aug; 279(35):37069.
Application：WB, Rat, PC-12 cells.
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NPDC-1, a regulator of neural cell proliferation and differentiation, interacts with E2F-1, reduces its binding to DNA and modulates its transcriptional activity.
Sansal I, Dupont E, Toru D, Evrard C, Rouget P.
Oncognen 2000 Oct; 19(43):5000.
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Subcellular localization of neural-specific NPDC-1 protein.
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