CTSL monoclonal antibody, clone AACD-3
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) HepG2, (2) NIH/3T3, and (3) PC-12 cell lysate.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human CTSL.
Immunogen
A synthetic peptide corresponding to human CTSL.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-200)
Immunofluorescence (1:50-200)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-200)
Western Blot (1:500-2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) HepG2, (2) NIH/3T3, and (3) PC-12 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — CTSL1
Entrez GeneID
1514Protein Accession#
P07711Gene Name
CTSL1
Gene Alias
CATL, CTSL, FLJ31037, MEP
Gene Description
cathepsin L1
Omim ID
116880Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000021601|OTTHUMP00000021602|OTTHUMP00000063566|cathepsin L|major excreted protein
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Interactome
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Pathway
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