Myod1 monoclonal antibody, clone 5.2F
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Specification
Product Description
Mouse monoclonal antibody raised agains recombinant Myod1.
Immunogen
Recombinant protein corresponding to mouse Myod1.
Host
Mouse
Reactivity
Chicken, Human, Mouse, Rat
Form
Liquid
Isotype
IgG2a
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western blot (1 ug/mL)
Immunoprecipitation (2 ug/mg of protein lysate)
Immunohistochemistry on frozen and formalin to fixed/paraffin to embedded tissues (2 to 4 ug/mL)
The optimal working dilution should be determined by the endStorage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining using Myod1 monoclonal antibody, clone 5.2F (Cat # MAB1962) on formalin fixed, paraffin embedded human rhabdomyosarcoma.Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Myod1
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Publication Reference
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Alpha 2a-interferon-induced differentiation of human alveolar rhabdomyosarcoma cells: correlation with down-regulation of the insulin-like growth factor type I receptor.
Thulasi R, Dias P, Houghton PJ, Houghton JA.
Cell Growth & Differentiation 1996 Apr; 7(4):531.
Application:ICC, WB-Ce, Human, Ah30, AS#23 cells.
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Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas.
Wesche WA, Fletcher CD, Dias P, Houghton PJ, Parham DM.
The American Journal of Surgical Pathology 1995 Mar; 19(3):261.
Application:IHC-Fr, Human, Rhabdomyosarcoma, Soft tissue scarcoma.
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Alpha 2a-interferon-induced differentiation of human alveolar rhabdomyosarcoma cells: correlation with down-regulation of the insulin-like growth factor type I receptor.
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