TUBB1 monoclonal antibody, clone CO-20
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) K562 cell lysate; (2) Jurkat cell lysate; (3) HeLa cell lysate; (4) 293T cell lysate with TUBB1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human tonsil with TUBB1 monoclonal antibody.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human TUBB1.
Immunogen
A synthetic peptide corresponding to human TUBB1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:100-1:200)
Immunofluorescence (1:100-1:200)
Immunohistochemistry (1:100-1:200)
Flow Cytometry (1:100)
Western Blot (1:3000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) K562 cell lysate; (2) Jurkat cell lysate; (3) HeLa cell lysate; (4) 293T cell lysate with TUBB1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human tonsil with TUBB1 monoclonal antibody.Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — TUBB1
Entrez GeneID
81027Protein Accession#
Q9H4B7Gene Name
TUBB1
Gene Alias
dJ543J19.4
Gene Description
tubulin, beta 1
Gene Ontology
HyperlinkGene Summary
Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM
Other Designations
OTTHUMP00000031411|beta tubulin 1, class VI
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Interactome
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Pathway
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Disease
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