Myog monoclonal antibody, clone F12B
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining using Myog monoclonal antibody, clone F12B (Cat # MAB1961) on formalin fixed, paraffin embedded human rhabdomyosarcoma.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Myog.
Immunogen
Recombinant protein corresponding to amino acids 30-224 of rat Myog.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western blot (1 ug/mL)
Immunoprecipitation (2 ug/mg of protein lysate)
Immunohistochemistry on formalin to fixed/paraffin to embedded tissues (1 to 2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining using Myog monoclonal antibody, clone F12B (Cat # MAB1961) on formalin fixed, paraffin embedded human rhabdomyosarcoma.Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Gel Supershift Assay
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Gene Info — Myog
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Publication Reference
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Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interactions.
Wright WE, Dac-Korytko I, Farmer K.
Developmental Genetics 1996 Jan; 19(2):131.
Application：Func, IF, IP, WB, Mouse, C2C12 cells.
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Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype.
Aurade F, Pinset C, Chafey P, Gros F, Montarras D.
Differentiation 1994 Feb; 55(3):185.
Application：WB-Ce, WB-Tr, Mouse, C3H10T1/2 cells.
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Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.
Wright WE, Sassoon DA, Lin VK.
Cell 1989 Feb; 56(4):607.
Application：IF, Mouse, C3H10T1/2 cells.
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Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interactions.
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