APOB monoclonal antibody, clone AOIH-1
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human APOB.
Immunogen
A synthetic peptide corresponding to human APOB.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:50)
Flow Cytometry (1:100)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of human serum lysate with APOB monoclonal antibody.Immunoprecipitation
Flow Cytometry
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Gene Info — APOB
Entrez GeneID
338Protein Accession#
P04114Gene Name
APOB
Gene Alias
FLDB
Gene Description
apolipoprotein B (including Ag(x) antigen)
Gene Ontology
HyperlinkGene Summary
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq
Other Designations
OTTHUMP00000115994|apoB-100|apoB-48|apolipoprotein B|apolipoprotein B48
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Interactome
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Disease
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