APAF1 monoclonal antibody, clone BHF-1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell lysate; (2) MCF-7 cell lysate with APAF1 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded mouse kidney with APAF1 monoclonal antibody.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human APAF1.
Immunogen
A synthetic peptide corresponding to human APAF1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) HeLa cell lysate; (2) MCF-7 cell lysate with APAF1 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded mouse kidney with APAF1 monoclonal antibody.Immunocytochemistry
Immunofluorescence
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Gene Info — APAF1
Entrez GeneID
317Protein Accession#
O14727Gene Name
APAF1
Gene Alias
CED4, DKFZp781B1145
Gene Description
apoptotic peptidase activating factor 1
Omim ID
602233Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
apoptotic protease activating factor 1
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Interactome
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Pathway
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Disease
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