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Last updated: 2020/11/22

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H2AFX monoclonal antibody, clone AbH41 

  • Catalog # : MAB19495
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against synthetic peptide of human H2AFX.
  • Immunogen:
  • A synthetic peptide corresponding to human H2AFX.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunohistochemistry (1:50~1:200)
    Immunoprecipitation (1:30)
    Western Blot (1:5000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Instruction:
  • Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent analysis of HeLa cells treated with H2O2, using H2AFX monoclonal antibody.
  • Immunoprecipitation
  • Application Image
  • Western Blot (Cell lysate)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 3014
  • Gene Name:
  • H2AFX
  • Gene Alias:
  • H2A.X,H2A/X,H2AX
  • Gene Description:
  • H2A histone family, member X
  • Gene Summary:
  • Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
  • Other Designations:
  • H2AX histone
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