NOX4 monoclonal antibody, clone E-14
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of JAR cell lysate with NOX4 monoclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rat kidney with NOX4 monoclonal antibody.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human NOX4.
Immunogen
A synthetic peptide corresponding to human NOX4.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of JAR cell lysate with NOX4 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded rat kidney with NOX4 monoclonal antibody.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — NOX4
Entrez GeneID
50507Protein Accession#
Q9NPH5Gene Name
NOX4
Gene Alias
KOX, KOX-1, RENOX
Gene Description
NADPH oxidase 4
Omim ID
605261Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants
Other Designations
kidney superoxide-producing NADPH oxidase|renal NAD(P)H-oxidase
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Interactome
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Disease
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