Acrolein monoclonal antibody, clone 2H2 (PerCP)
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More Files
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Immunocytochemistry
Immunocytochemical staining of HEK293 with Acrolein monoclonal antibody, clone 2H2 (PerCP) (Cat # MAB19243). (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) Acrolein Antibody. (G, H) Composite. (A,C,E,G) Untreated and (B,D,F,H) Cells cultured overnight with 50 uM H2O2.
Flow Cytometry
Flow cytometric analysis of SH-SY5Y with Acrolein monoclonal antibody, clone 2H2 (PerCP) (Cat # MAB19243).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of Acrolein.
Immunogen
Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
Host
Mouse
Form
Liquid
Conjugation
PerCP
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
ELISA (1:1000)
Flow Cytometry
Immunocytochemistry (1:50)
Immunofluorescence (1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunocytochemical staining of HEK293 with Acrolein monoclonal antibody, clone 2H2 (PerCP) (Cat # MAB19243). (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) Acrolein Antibody. (G, H) Composite. (A,C,E,G) Untreated and (B,D,F,H) Cells cultured overnight with 50 uM H2O2.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of SH-SY5Y with Acrolein monoclonal antibody, clone 2H2 (PerCP) (Cat # MAB19243).
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