Immunofluorescence microscopy of CENPE monoclonal antibody, clone 1H12 (Cat # MAB1924) was used to detect CENPE protein visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A) . Interphase cells show no discrete staining (bottom left, panel B) . HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. AlexaFluor 555 conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green) . Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.
Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq
Centromere autoantigen E (312kD),centromere protein E,centromere protein E (312kD),kinesin family member 10