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Last updated: 2016/10/23
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CENPE monoclonal antibody, clone 1H12

  • Catalog # : MAB1924
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant.
  • Immunogen:
  • Recombinant protein corresponding to full length human CENPE.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • Western Blot (1:500-1:2000)
    Immunoprecipitation (1:200)
    Immunofluorescence (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence microscopy of CENPE monoclonal antibody, clone 1H12 (Cat # MAB1924) was used to detect CENPE protein visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A) .
    Interphase cells show no discrete staining (bottom left, panel B) .
    HeLa cells were fixed in paraformaldehyde and stained using this primary antibody.
    AlexaFluor 555 conjugated anti-Mouse antibody (red) was used for detection.
    DNA was stained using bis-benzimide (DAPI) (green) .
    Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.
  • Immunoprecipitation
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1062
  • Protein Accession#:
  • P49840;NP_063937
  • Gene Name:
  • Gene Alias:
  • KIF10
  • Gene Description:
  • centromere protein E, 312kDa
  • Gene Summary:
  • Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq
  • Other Designations:
  • Centromere autoantigen E (312kD),centromere protein E,centromere protein E (312kD),kinesin family member 10
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