CDC2 monoclonal antibody, clone POH-1 (Cat # MAB1921) is shown to detect human CDC2 by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of CDC2 monoclonal antibody, clone POH-1 (Cat # MAB1921) at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5,000. A doublet band corresponding to human CDC2 is detected at ~34 KDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human CDC2. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.
Western Blot (Cell lysate)
CDC2 monoclonal antibody, clone POH-1 (Cat # MAB1921) was used to detect human CDC2 by western blot in untreated (control) and drug treated (10 uM genistein) lysates of MCF-7 cells. Very strong detection occurs using a 1:1,000 dilution. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
OTTHUMP00000019660,cell cycle controller CDC2,cell division control protein 2 homolog,cell division cycle 2 protein,cyclin-dependent kinase 1,p34 protein kinase