STAT1 (phospho S727) monoclonal antibody, clone C6
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More Files
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human STAT1.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser727 of human phospho STAT1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1k
Recommend Usage
Flow Cytometry (1 ug/mL-0.001 ug/mL )
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.1% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of U937 cell extract (1) treated with IL-4, (2) untreated with IL-4.Flow Cytometry
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNΑ IL4 and pervanadate (green) using STAT1 (phospho S727) monoclonal antibody. -
Gene Info — STAT1
Entrez GeneID
6772Protein Accession#
AAH02704Gene Name
STAT1
Gene Alias
DKFZp686B04100, ISGF-3, STAT91
Gene Description
signal transducer and activator of transcription 1, 91kDa
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000165047|signal transducer and activator of transcription 1|signal transducer and activator of transcription-1|transcription factor ISGF-3 components p91/p84
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Interactome
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Disease
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