RPS6 (phospho S235/S236) monoclonal antibody, clone R3A2 (SureLight 488)
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More Files
- More Functions
Flow Cytometry
Flow cytometric analysis of U937 cells unstained U0126 plus SB20350 cells (blue) or stained and treated with U0126 plus SB20350 (red) or treated with TPA plus calyculin A (green) using RPS6 (phospho S235/S236) monoclonal antibody (SureLight 488).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human RPS6.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S235/S236 of human RPS6.
Host
Rabbit
Reactivity
Human
Form
Liquid
Conjugation
SureLight 488
Purification
Protein A/G Purification
Isotype
IgG1k
Recommend Usage
Flow Cytometry (5 uL/106 cells or 0.05 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.2% BSA, 0.09% sodium azide).
Storage Instruction
Store at 2-8°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Flow cytometric analysis of U937 cells unstained U0126 plus SB20350 cells (blue) or stained and treated with U0126 plus SB20350 (red) or treated with TPA plus calyculin A (green) using RPS6 (phospho S235/S236) monoclonal antibody (SureLight 488). -
Gene Info — RPS6
Entrez GeneID
6194Gene Name
RPS6
Gene Alias
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Gene Description
ribosomal protein S6
Omim ID
180460Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
40S ribosomal protein S6|OTTHUMP00000021120|phosphoprotein NP33
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Interactome
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Pathway
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