MAP2K1 (phospho S298) monoclonal antibody, clone H8
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human MAP2K1.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S298 of human MAP2K1.
Host
Rabbit
Reactivity
Human, Rat
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.1% BSA and 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of C6 cell lysate with MAP2K1 (phospho S298) monoclonal antibody, clone H8 (Cat # MAB18936). Lane 1: treated or Lane 2: untreated with TPA.Flow Cytometry
Flow cytometric analysis of HeLa cells with MAP2K1 (phospho S298) monoclonal antibody, clone H8 (Cat # MAB18936). Secondary antibody only negative control (blue) or treated with imatinib (red) or treated with pervanadate (green). -
Gene Info — MAP2K1
Entrez GeneID
5604Gene Name
MAP2K1
Gene Alias
MAPKK1, MEK1, MKK1, PRKMK1
Gene Description
mitogen-activated protein kinase kinase 1
Omim ID
176872Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
Other Designations
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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