LCK (phospho Y505) monoclonal antibody, clone A3
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with LCK (phospho Y505) monoclonal antibody, clone A3 (Cat # MAB18923). Lane 1: treated or Lane 2: untreated with 200 nM calyculin A for 30min.
Flow Cytometry
Flow cytometric analysis of Daudi cells with LCK (phospho Y505) monoclonal antibody, clone A3 (Cat # MAB18923). Secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human LCK.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Y505 of human LCK.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.1% BSA and 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with LCK (phospho Y505) monoclonal antibody, clone A3 (Cat # MAB18923). Lane 1: treated or Lane 2: untreated with 200 nM calyculin A for 30min.Flow Cytometry
Flow cytometric analysis of Daudi cells with LCK (phospho Y505) monoclonal antibody, clone A3 (Cat # MAB18923). Secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green). -
Gene Info — LCK
Entrez GeneID
3932Gene Name
LCK
Gene Alias
YT16, p56lck, pp58lck
Gene Description
lymphocyte-specific protein tyrosine kinase
Omim ID
153390Gene Ontology
HyperlinkGene Summary
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq
Other Designations
T-lymphocyte specific protein tyrosine kinase p56lck|p56(LSTRA) protein-tyrosine kinase|protein tyrosine kinase|proto-oncogene tyrosine-protein kinase LCK
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Interactome
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Pathway
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Disease
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